Rv0620 Galactokinase (EC 2.7.1.6)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0620 galK Galactokinase (EC 2.7.1.6) CDS 712716 713807 + 1 092 363 FALSE

Rv0620 (Galactokinase (EC 2.7.1.6)) is predicted to be co-regulated in modules bicluster_0429 with residual 0.52 and bicluster_0557 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1.40 and 2,100.00 for bicluster_0429 and 7,700.00 and 9,400.00 for bicluster_0557 respectively.

These modules are enriched for following go terms: transferase activity, transferring one-c... chromosome segregation, cell cycle, cell division.

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0429
e.value: 
1.4
Motif Bicluster: 
e.value: 
2100
Motif Bicluster: 
0.52
bicluster_0557
e.value: 
7700
Motif Bicluster: 
e.value: 
9400
Motif Bicluster: 
0.50
Product (LegacyBRC) Product (RefSeq)
Galactokinase galactokinase
Operon # Operon
416 Rv0618 - Rv0619 - Rv0620
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Galactokinase Amino sugar and nucleotide sugar metabolism, Galactose metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Galactose metabolism

14
Total items in this category:  

KEGG

Amino sugar and nucleotide sugar metabolism

28
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607760 NP_215134.1 Run
GO:0004335

galactokinase activity

galactokinase activity

Details: 
Catalysis of the reaction: D-galactose + ATP = alpha-D-galactose 1-phosphate + ADP + 2 H(+).
GO Category: 
molecular_function
1
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.100000 0.06

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.