These modules are enriched for following go terms: response to stress, cellular response to stimulus, single-organism cellular process, nucleic acid metabolic process, four-way junction helicase activity, endodeoxyribonuclease activity, hydrolase activity, acting on ester bond....
This gene is found to be for growth on cholesterol.
|Gene Target||Differential Expression||Distance||Expression||pvalue||Type|
|ChipSeq TF||Differential Expression||Distance||Expression||pvalue||Type|
|Product (LegacyBRC)||Product (RefSeq)|
|Putative uncharacterized protein|
|BioCyc Gene Page||Cellular Overview Map|
|t-test p-value||Cholesterol/Glycerol Ratio|
How essentiality calculations were done?
The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.
reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.