Rv0634c

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0634c CDS 730320 731033 - 714 237 FALSE

Rv0634c () is predicted to be co-regulated in modules bicluster_0057 with residual 0.63 and bicluster_0540 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.02 for bicluster_0057 and 2,700.00 and 15,000.00 for bicluster_0540 respectively.

These modules are enriched for following go terms: tetrapyrrole metabolic process, tetrapyrrole biosynthetic process, vitamin metabolic process, water-soluble vitamin metabolic process, vitamin biosynthetic process, water-soluble vitamin biosynthetic proce... .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
Possible oxidoreductase subunit
Repressed -135 -0.79 0.00000000108 Primary.TSS
Two component transcriptional regulatory protein DevR
No -4 -0.25 0.0215182 Primary.TSS
Transcriptional regulator, TetR family
Repressed -134 -0.7 0.0000000569 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0057
e.value: 
0.00017
Motif Bicluster: 
e.value: 
0.015
Motif Bicluster: 
0.63
bicluster_0540
e.value: 
2700
Motif Bicluster: 
e.value: 
15000
Motif Bicluster: 
0.52
Product (LegacyBRC) Product (RefSeq)
POSSIBLE GLYOXALASE II [HYDROXYACYLGLUTATHIONE HYDROLASE] [GLX II] glyoxalase II
Operon # Operon
428
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics
Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Pyruvate metabolism

40
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607774 NP_215148.1 Run
GO:0004416

hydroxyacylglutathione hydrolase activity

hydroxyacylglutathione hydrolase activity

Details: 
Catalysis of the reaction: (S)-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.
GO Category: 
molecular_function
3
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.130000 0.67

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: