Rv0635

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0635 hadA CDS 731930 732406 + 477 158 FALSE

Rv0635 () is predicted to be co-regulated in modules bicluster_0092 with residual 0.47 and bicluster_0586 with residual 0.63.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 490.00 and 6,500.00 for bicluster_0092 and 1,800.00 and 5,500.00 for bicluster_0586 respectively.

These modules are enriched for following go terms: nucleic acid metabolic process, heterocyclic compound binding, organic cyclic compound binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
UPF0336 protein Rv0635_MT0664 (3R)-hydroxyacyl-ACP dehydratase subunit HadA
Operon # Operon
429 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics
Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607775 NP_215149.1 Run
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0019171

3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity

3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity

Details: 
Catalysis of the reaction: a (3R)-3-hydroxyacyl-[acyl-carrier protein] = H2O + a trans-delta2-enoyl-acyl-[acyl-carrier protein].
GO Category: 
molecular_function
5
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: