Rv0688 Ferredoxin reductase

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0688 Ferredoxin reductase CDS 787940 789160 + 1 221 406 FALSE

Rv0688 (Ferredoxin reductase) is predicted to be co-regulated in modules bicluster_0098 with residual 0.64 and bicluster_0569 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0098 and 500.00 and 460.00 for bicluster_0569 respectively.

These modules are enriched for following go terms: purine-nucleoside phosphorylase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 05:58
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18527 MT0716 2468
Product (LegacyBRC) Product (RefSeq)
PUTATIVE FERREDOXIN REDUCTASE putative ferredoxin reductase
Operon # Operon
461 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607828 NP_215202.1 Run
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0008860

ferredoxin-NAD+ reductase activity

ferredoxin-NAD+ reductase activity

Details: 
Catalysis of the reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0009055

electron carrier activity

electron carrier activity

Details: 
Any molecular entity that serves as an electron acceptor and electron donor in an electron transport system.
GO Category: 
molecular_function
10
Total items in this category:  
GO:0050660

flavin adenine dinucleotide binding

flavin adenine dinucleotide binding

Details: 
Interacting selectively and non-covalently with FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2.
GO Category: 
molecular_function
10
Total items in this category:  
GO:0051287

NAD binding

NAD binding

Details: 
Interacting selectively and non-covalently with nicotinamide adenine dinucleotide, a coenzyme involved in many redox and biosynthetic reactions; binding may be to either the oxidized form, NAD+, or the reduced form, NADH.
GO Category: 
molecular_function
3
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.840000 1.92

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: