Rv0694 Mycofactocin system heme/flavin dehydrogenase

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0694 lldD1 Mycofactocin system heme/flavin dehydrogenase CDS 793335 794525 + 1 191 396 FALSE

Rv0694 (Mycofactocin system heme/flavin dehydrogenase) is predicted to be co-regulated in modules bicluster_0157 with residual 0.53 and bicluster_0396 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0157 and 0.01 and 9.30 for bicluster_0396 respectively.

These modules are enriched for following go terms: organic hydroxy compound transport, tetracycline transport, antibiotic transport, toxin transport, drug transport, response to drug, tetracycline transporter activity, drug:hydrogen antiporter activity, tetracycline:hydrogen antiporter activit..., toxin transporter activity, antibiotic transporter activity, organic hydroxy compound transmembrane t..., solute:cation antiporter activity, solute:hydrogen antiporter activity, antiporter activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
POSSIBLE L-LACTATE DEHYDROGENASE [CYTOCHROME] LLDD1 L-lactate dehydrogenase (cytochrome) LldD1
Operon # Operon
464 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Pyruvate metabolism

40
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607834 NP_215208.1 Run
GO:0004460

L-lactate dehydrogenase (cytochrome) activity

L-lactate dehydrogenase (cytochrome) activity

Details: 
Catalysis of the reaction: (S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.010000 0.06

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: