Rv0715 LSU ribosomal protein L24p (L26e)
This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 82.00 and 1,100.00 for bicluster_0197 and 1,100.00 and 5,100.00 for bicluster_0333 respectively.
These modules are enriched for following go terms: translational elongation, cytoplasm, intracellular, translation elongation factor activity, translation factor activity, nucleic aci... regulation of transcription, DNA-depende..., regulation of RNA metabolic process, regulation of RNA biosynthetic process, regulation of biosynthetic process, regulation of gene expression, regulation of macromolecule biosynthetic..., regulation of cellular biosynthetic proc..., regulation of cellular macromolecule bio..., regulation of nucleobase-containing comp..., regulation of nitrogen compound metaboli..., regulation of cellular metabolic process, regulation of macromolecule metabolic pr..., regulation of primary metabolic process, transcription, DNA-dependent, regulation of metabolic process, RNA biosynthetic process, pyruvate dehydrogenase activity, pyruvate dehydrogenase (acetyl-transferr..., oxidoreductase activity, acting on the a....
This gene is found to be for growth on cholesterol.
|Product (LegacyBRC)||Product (RefSeq)|
|50S ribosomal protein L24||50S ribosomal protein L24|
|475||- - -|
|BioCyc Gene Page||Cellular Overview Map|
|t-test p-value||Cholesterol/Glycerol Ratio|
How essentiality calculations were done?
The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.
reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.