Rv0753c Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0753c mmsA Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) CDS 844421 845953 - 1 533 510 FALSE

Rv0753c (Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)) is predicted to be co-regulated in modules bicluster_0035 with residual 0.52 and bicluster_0401 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 130.00 for bicluster_0035 and 100.00 and 180.00 for bicluster_0401 respectively.

These modules are enriched for following go terms: hydroxymethyl-, formyl- and related tran... .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE MMSA [METHYLMALONIC ACID SEMIALDEHYDE DEHYDROGENASE] [MMSDH] methylmalonate-semialdehyde dehydrogenase
Operon # Operon
498 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Inositol phosphate metabolism

9
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607893 NP_215267.1 Run
GO:0004491

methylmalonate-semialdehyde dehydrogenase (acylating) activity

methylmalonate-semialdehyde dehydrogenase (acylating) activity

Details: 
Catalysis of the reaction: 2-methyl-3-oxopropanoate + CoA + NAD+ = propanoyl-CoA + CO2 + NADH + H+.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.240000 1.14

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: