Rv0773c Gamma-glutamyltranspeptidase (EC 2.3.2.2)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0773c ggtA Gamma-glutamyltranspeptidase (EC 2.3.2.2) CDS 865851 867389 - 1 539 512 FALSE

Rv0773c (Gamma-glutamyltranspeptidase (EC 2.3.2.2)) is predicted to be co-regulated in modules bicluster_0081 with residual 0.55 and bicluster_0233 with residual 0.48.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.53 and 19,000.00 for bicluster_0081 and 24,000.00 and 11,000.00 for bicluster_0233 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.369 867434 867389
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator kstR (Rv3574), TetR family
No -146 0.01 0.969528 CDS
Transcriptional regulator, TetR family
No -16 0.13 0.619075 CDS
Motif 1 Motif 2 Residual
bicluster_0081
e.value: 
0.53
Motif Bicluster: 
e.value: 
19000
Motif Bicluster: 
0.55
bicluster_0233
e.value: 
24000
Motif Bicluster: 
e.value: 
11000
Motif Bicluster: 
0.48
Product (LegacyBRC) Product (RefSeq)
PROBABLE BIFUNCTIONAL ACYLASE GGTA: CEPHALOSPORIN ACYLASE [GL-7ACA ACYLASE] + GAMMA-GLUTAMYLTRANSPEPTIDASE [GGT] bifunctional cephalosporin acylase/gamma-glutamyltranspeptidase
Operon # Operon
510
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Taurine and hypotaurine metabolism

7
Total items in this category:  

KEGG

Selenocompound metabolism

40
Total items in this category:  

KEGG

Cyanoamino acid metabolism

12
Total items in this category:  

KEGG

Glutathione metabolism

14
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607913 NP_215287.1 Run
GO:0003840

gamma-glutamyltransferase activity

gamma-glutamyltransferase activity

Details: 
Catalysis of the reaction: (5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl-amino acid.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0006751

glutathione catabolic process

glutathione catabolic process

Details: 
The chemical reactions and pathways resulting in the breakdown of glutathione, the tripeptide glutamylcysteinylglycine, which acts as a coenzyme for some enzymes and as an antioxidant in the protection of sulfhydryl groups in enzymes and other proteins.
GO Category: 
biological_process
1
Total items in this category:  
GO:0034722

gamma-glutamyl-peptidase activity

gamma-glutamyl-peptidase activity

Details: 
Catalysis of the cleavage of a gamma-linked glutamate bond.
GO Category: 
molecular_function
1
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.440000 1.45

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: