Rv0774c Gamma-glutamyltranspeptidase (EC 2.3.2.2)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0774c Gamma-glutamyltranspeptidase (EC 2.3.2.2) CDS 867440 868351 - 912 303 FALSE

Rv0774c (Gamma-glutamyltranspeptidase (EC 2.3.2.2)) is predicted to be co-regulated in modules bicluster_0279 with residual 0.52 and bicluster_0345 with residual 0.48.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.16 and 260.00 for bicluster_0279 and 3,300.00 and 4,600.00 for bicluster_0345 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-0.673 12 868339 868339 868351
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 5 of 5
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, MarR family
Repressed 41 -2.14 0 Internal.TSS
No -6 -0.29 0.183921 Internal.TSS
Transcriptional regulator, MarR family
Repressed 29 -2.14 0 CDS
No 52 -0.29 0.183921 Antisense.TSS
Two component transcriptional regulatory protein DevR
No 9 -0.1 0.325028 CDS
Motif 1 Motif 2 Residual
bicluster_0279
e.value: 
0.16
Motif Bicluster: 
e.value: 
260
Motif Bicluster: 
0.52
bicluster_0345
e.value: 
3300
Motif Bicluster: 
e.value: 
4600
Motif Bicluster: 
0.48
Product (LegacyBRC) Product (RefSeq)
PROBABLE CONSERVED EXPORTED PROTEIN
Operon # Operon
511
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116780 NP_215288.2 Run
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.400000 1.71

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: