Rv0805 3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0805 3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17) CDS 898831 899787 + 957 318 FALSE

Rv0805 (3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17)) is predicted to be co-regulated in modules bicluster_0400 with residual 0.51 and bicluster_0593 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 0.12 for bicluster_0400 and 4,900.00 and 7,400.00 for bicluster_0593 respectively.

These modules are enriched for following go terms: carboxypeptidase activity, thiosulfate sulfurtransferase activity, unfolded protein binding, exopeptidase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
531
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

3',5'-cyclic-nucleotide phosphodiesterase Purine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607945 NP_215320.1 Run
GO:0004115

3',5'-cyclic-AMP phosphodiesterase activity

3',5'-cyclic-AMP phosphodiesterase activity

Details: 
Catalysis of the reaction: adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005506

iron ion binding

iron ion binding

Details: 
Interacting selectively and non-covalently with iron (Fe) ions.
GO Category: 
molecular_function
13
Total items in this category:  
GO:0008199

ferric iron binding

ferric iron binding

Details: 
Interacting selectively and non-covalently with ferric iron, Fe(III).
GO Category: 
molecular_function
1
Total items in this category:  
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
GO:0030145

manganese ion binding

manganese ion binding

Details: 
Interacting selectively and non-covalently with manganese (Mn) ions.
GO Category: 
molecular_function
31
Total items in this category:  
GO:0042301

phosphate ion binding

phosphate ion binding

Details: 
Interacting selectively and non-covalently with phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.010000 0.11

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: