Rv0855 Alpha-methylacyl-CoA racemase (EC 5.1.99.4)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0855 far Alpha-methylacyl-CoA racemase (EC 5.1.99.4) CDS 951632 952711 + 1 080 359 FALSE

Rv0855 (Alpha-methylacyl-CoA racemase (EC 5.1.99.4)) is predicted to be co-regulated in modules bicluster_0048 with residual 0.53 and bicluster_0591 with residual 0.62.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.40 and 37.00 for bicluster_0048 and 56.00 and 610.00 for bicluster_0591 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 08:59
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14779 MT0878 138
Product (LegacyBRC) Product (RefSeq)
PROBABLE FATTY-ACID-CoA RACEMASE FAR fatty-acid-CoA racemase
Operon # Operon
572 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Alpha-methylacyl-CoA racemase Primary bile acid biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607995 NP_215370.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.790000 2.28

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: