Rv0860 Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0860 fadB Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.... CDS 956293 958455 + 2 163 720 FALSE

Rv0860 (Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)) is predicted to be co-regulated in modules bicluster_0402 with residual 0.39 and bicluster_0475 with residual 0.40.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 650.00 for bicluster_0402 and 0.00 and 9.10 for bicluster_0475 respectively.

These modules are enriched for following go terms: fatty acid synthase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 08:59
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17878 MT0883 443
Product (LegacyBRC) Product (RefSeq)
PROBABLE FATTY OXIDATION PROTEIN FADB fatty oxidation protein FadB
Operon # Operon
575 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

3-hydroxyacyl-CoA dehydrogenase Lysine degradation, Fatty acid metabolism, beta-Alanine metabolism, Fatty acid elongation in mitochondria, Butanoate metabolism, Valine, leucine and isoleucine degradation, 1,4-Dichlorobenzene degradation, Fatty acid metabolism, Fatty acid metabolism, 2,4-Dichlorobenzoate degradation, Geraniol degradation, Fatty acid elongation in mitochondria, Valine, leucine and isoleucine degradation, Caprolactam degradation, Reductive carboxylate cycle (CO2 fixation), Tryptophan metabolism, Caprolactam degradation, Lysine degradation, Limonene and pinene degradation, Benzoate degradation via hydroxylation, Tryptophan metabolism, Propanoate metabolism, Butanoate metabolism, Primary bile acid biosynthesis, alpha-Linolenic acid metabolism, Geraniol degradation, Butanoate metabolism

PATRIC

3-hydroxybutyryl-CoA epimerase Lysine degradation, Fatty acid metabolism, beta-Alanine metabolism, Fatty acid elongation in mitochondria, Butanoate metabolism, Valine, leucine and isoleucine degradation, 1,4-Dichlorobenzene degradation, Fatty acid metabolism, Fatty acid metabolism, 2,4-Dichlorobenzoate degradation, Geraniol degradation, Fatty acid elongation in mitochondria, Valine, leucine and isoleucine degradation, Caprolactam degradation, Reductive carboxylate cycle (CO2 fixation), Tryptophan metabolism, Caprolactam degradation, Lysine degradation, Limonene and pinene degradation, Benzoate degradation via hydroxylation, Tryptophan metabolism, Propanoate metabolism, Butanoate metabolism, Primary bile acid biosynthesis, alpha-Linolenic acid metabolism, Geraniol degradation, Butanoate metabolism

PATRIC

Enoyl-CoA hydratase Lysine degradation, Fatty acid metabolism, beta-Alanine metabolism, Fatty acid elongation in mitochondria, Butanoate metabolism, Valine, leucine and isoleucine degradation, 1,4-Dichlorobenzene degradation, Fatty acid metabolism, Fatty acid metabolism, 2,4-Dichlorobenzoate degradation, Geraniol degradation, Fatty acid elongation in mitochondria, Valine, leucine and isoleucine degradation, Caprolactam degradation, Reductive carboxylate cycle (CO2 fixation), Tryptophan metabolism, Caprolactam degradation, Lysine degradation, Limonene and pinene degradation, Benzoate degradation via hydroxylation, Tryptophan metabolism, Propanoate metabolism, Butanoate metabolism, Primary bile acid biosynthesis, alpha-Linolenic acid metabolism, Geraniol degradation, Butanoate metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid metabolism

51
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Geraniol degradation

52
Total items in this category:  

KEGG

Lysine degradation

46
Total items in this category:  

KEGG

Tryptophan metabolism

47
Total items in this category:  

KEGG

beta-Alanine metabolism

37
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  

KEGG

Carbon fixation pathways in prokaryotes

42
Total items in this category:  

KEGG

Limonene and pinene degradation

65
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608000 NP_215375.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.140000 2.23

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: