Rv0861c DNA repair helicase

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0861c ercc3 DNA repair helicase CDS 958523 960151 - 1 629 542 FALSE

Rv0861c (DNA repair helicase) is predicted to be co-regulated in modules bicluster_0189 with residual 0.44 and bicluster_0359 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 13,000.00 and 13,000.00 for bicluster_0189 and 0.00 and 0.00 for bicluster_0359 respectively.

These modules are enriched for following go terms: glycerol metabolic process, alditol metabolic process, polyol metabolic process, alcohol metabolic process, organic hydroxy compound metabolic proce..., aspartic-type endopeptidase activity, aspartic-type peptidase activity, ATP-dependent DNA helicase activity, DNA-dependent ATPase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-1.8 161 960333 960172 960151
Product (LegacyBRC) Product (RefSeq)
PROBABLE DNA HELICASE ERCC3 DNA helicase ErcC3
Operon # Operon
576
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Nucleotide excision repair

14
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608001 NP_215376.1 Run
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.770000 1.20

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: