Rv0892 Cyclohexanone monooxygenase (EC 1.14.13.22)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0892 Cyclohexanone monooxygenase (EC 1.14.13.22) CDS 993853 995340 + 1 488 495 FALSE

Rv0892 (Cyclohexanone monooxygenase (EC 1.14.13.22)) is predicted to be co-regulated in modules bicluster_0071 with residual 0.59 and bicluster_0153 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.04 and 0.76 for bicluster_0071 and 0.01 and 20.00 for bicluster_0153 respectively.

These modules are enriched for following go terms: nucleotide metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 08:59
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18280 MT0916 1922
Product (LegacyBRC) Product (RefSeq)
Uncharacterized monooxygenase Rv0892_MT0916 monooxygenase
Operon # Operon
597
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Cyclohexanone monooxygenase Caprolactam degradation
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Histidine metabolism

40
Total items in this category:  

KEGG

Chlorocyclohexane and chlorobenzene degradation

7
Total items in this category:  

KEGG

Bisphenol degradation

35
Total items in this category:  

KEGG

Toluene degradation

13
Total items in this category:  

KEGG

Polycyclic aromatic hydrocarbon degradation

47
Total items in this category:  

KEGG

Naphthalene degradation

40
Total items in this category:  

KEGG

Aminobenzoate degradation

73
Total items in this category:  

KEGG

Limonene and pinene degradation

65
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608032 NP_215407.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.240000 2.19

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: