Rv0897c Beta-carotene ketolase (EC 1.14.-.-)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0897c Beta-carotene ketolase (EC 1.14.-.-) CDS 1000808 1002415 - 1 608 535 FALSE

Rv0897c (Beta-carotene ketolase (EC 1.14.-.-)) is predicted to be co-regulated in modules bicluster_0067 with residual 0.54 and bicluster_0537 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 180.00 and 34.00 for bicluster_0067 and 0.00 and 0.04 for bicluster_0537 respectively.

These modules are enriched for following go terms: response to stress, cellular response to stimulus, single-organism cellular process, nucleic acid metabolic process, four-way junction helicase activity, endodeoxyribonuclease activity, hydrolase activity, acting on ester bond....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv0897c_MT0921 oxidoreductase
Operon # Operon
602 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608037 NP_215412.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.100000 1.00

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: