Rv0907 Beta-lactamase (EC 3.5.2.6)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0907 Beta-lactamase (EC 3.5.2.6) CDS 1010136 1011734 + 1 599 532 FALSE

Rv0907 (Beta-lactamase (EC 3.5.2.6)) is predicted to be co-regulated in modules bicluster_0093 with residual 0.41 and bicluster_0215 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,600.00 and 7,900.00 for bicluster_0093 and 13.00 and 140.00 for bicluster_0215 respectively.

These modules are enriched for following go terms: O-methyltransferase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-0.746 1064 1011200 1010313 1010136
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
607 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Beta-lactamase Penicillin and cephalosporin biosynthesis, beta-Lactam resistance
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608047 NP_215422.1 Run
GO:0008800

beta-lactamase activity

beta-lactamase activity

Details: 
Catalysis of the reaction: a beta-lactam + H2O = a substituted beta-amino acid.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.920000 1.36

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: