Rv0932c Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0932c pstS2 Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1) CDS 1039936 1041048 - 1 113 370 FALSE

Rv0932c (Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1)) is predicted to be co-regulated in modules bicluster_0142 with residual 0.59 and bicluster_0480 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 420.00 and 7,300.00 for bicluster_0142 and 0.30 and 53.00 for bicluster_0480 respectively.

These modules are enriched for following go terms: DNA topological change, glutamate metabolic process, dicarboxylic acid biosynthetic process, DNA conformation change, DNA topoisomerase activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 08:59
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14995 MT0959 463
Product (LegacyBRC) Product (RefSeq)
Phosphate-binding protein pstS 2 periplasmic phosphate-binding lipoprotein PSTS2 (PBP-2) (PSTS2)
Operon # Operon
621 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

ABC transporters

57
Total items in this category:  

KEGG

Two-component system

53
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116800 YP_177769.1 Run
GO:0006810

transport

transport

Details: 
The directed movement of substances (such as macromolecules, small molecules, ions) into, out of or within a cell, or between cells, or within a multicellular organism by means of some agent such as a transporter or pore.
GO Category: 
biological_process
28
Total items in this category:  
GO:0005215

transporter activity

transporter activity

Details: 
Enables the directed movement of substances (such as macromolecules, small molecules, ions) into, out of or within a cell, or between cells.
GO Category: 
molecular_function
27
Total items in this category:  
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0006817

phosphate ion transport

phosphate ion transport

Details: 
The directed movement of phosphate into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
GO Category: 
biological_process
4
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.690000 1.20

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: