Rv0952 Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0952 sucD Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5) CDS 1063140 1064051 + 912 303 FALSE

Rv0952 (Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5)) is predicted to be co-regulated in modules bicluster_0295 with residual 0.48 and bicluster_0301 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2.70 and 5,200.00 for bicluster_0295 and 0.00 and 4.40 for bicluster_0301 respectively.

These modules are enriched for following go terms: organonitrogen compound biosynthetic pro..., organonitrogen compound metabolic proces..., NAD biosynthetic process, nicotinamide nucleotide biosynthetic pro..., NAD metabolic process, pyridine nucleotide biosynthetic process, acetolactate synthase activity, transferase activity, transferring aldeh... divalent metal ion transport, divalent inorganic cation transport, transition metal ion transport, divalent inorganic cation transmembrane ....

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-1.414 1063149 1063140
Product (LegacyBRC) Product (RefSeq)
Succinyl-CoA ligase [ADP-forming] subunit alpha succinyl-CoA synthetase subunit alpha
Operon # Operon
635 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Citrate cycle (TCA cycle)

35
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

C5-Branched dibasic acid metabolism

9
Total items in this category:  

KEGG

Carbon fixation pathways in prokaryotes

42
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608092 NP_215467.1 Run
GO:0004775

succinate-CoA ligase (ADP-forming) activity

succinate-CoA ligase (ADP-forming) activity

Details: 
Catalysis of the reaction: ATP + succinate + CoA = ADP + succinyl-CoA + phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: