Rv0981 Two-component response regulator

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0981 mprA Two-component response regulator CDS 1096816 1097508 + 693 230 TRUE

Rv0981 (Two-component response regulator) is predicted to be co-regulated in modules bicluster_0017 with residual 0.60 and bicluster_0555 with residual 0.43.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.25 and 10.00 for bicluster_0017 and 480.00 and 33,000.00 for bicluster_0555 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 6 of 6
Gene Target Differential Expression Distance Expression pvalue Type
PROBABLE CONSERVED INTEGRAL MEMBRANE PROTEIN
No 52 -0.04 0.924107 Antisense.TSS
PPE family protein
No 5 0.01 0.990733 Internal.TSS
PPE family protein
No 48 0.01 0.990733 Internal.TSS
PE family protein
No -139 -0.3 0.501392 Primary.TSS
No -139 -0.13 0.838053 Internal.TSS
FIG035174: membrane protein
No -80 -0.21 0.434962 Primary.TSS
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, AsnC family
No -17 -0.45 0.0685618 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0017
e.value: 
0.25
Motif Bicluster: 
e.value: 
10
Motif Bicluster: 
0.60
bicluster_0555
e.value: 
480
Motif Bicluster: 
e.value: 
33000
Motif Bicluster: 
0.43
Product (LegacyBRC) Product (RefSeq)
Response regulator mprA two component response transcriptional regulatory protein MprA
Operon # Operon
655 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Two-component system

53
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608121 NP_215496.1 Run
GO:0008150

biological_process

biological_process

Details: 
Any process specifically pertinent to the functioning of integrated living units: cells, tissues, organs, and organisms. A process is a collection of molecular events with a defined beginning and end.
GO Category: 
biological_process
66
Total items in this category:  
GO:0008150

biological_process

biological_process

Details: 
Any process specifically pertinent to the functioning of integrated living units: cells, tissues, organs, and organisms. A process is a collection of molecular events with a defined beginning and end.
GO Category: 
biological_process
66
Total items in this category:  
GO:0000160

two-component signal transduction system (phosphorelay)

two-component signal transduction system (phosphorelay)

Details: 
A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.
GO Category: 
biological_process
4
Total items in this category:  
GO:0003677

DNA binding

DNA binding

Details: 
Any molecular function by which a gene product interacts selectively with DNA (deoxyribonucleic acid).
GO Category: 
molecular_function
37
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0006950

response to stress

response to stress

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a disturbance in organismal or cellular homeostasis, usually, but not necessarily, exogenous (e.g. temperature, humidity, ionizing radiation).
GO Category: 
biological_process
9
Total items in this category:  
GO:0045892

negative regulation of transcription, DNA-dependent

negative regulation of transcription, DNA-dependent

Details: 
Any process that stops, prevents, or reduces the frequency, rate or extent of cellular DNA-dependent transcription.
GO Category: 
biological_process
10
Total items in this category:  
GO:0006355

regulation of transcription, DNA-dependent

regulation of transcription, DNA-dependent

Details: 
Any process that modulates the frequency, rate or extent of cellular DNA-dependent transcription.
GO Category: 
biological_process
15
Total items in this category:  
GO:0045893

positive regulation of transcription, DNA-dependent

positive regulation of transcription, DNA-dependent

Details: 
Any process that activates or increases the frequency, rate or extent of cellular DNA-dependent transcription.
GO Category: 
biological_process
14
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1426596 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426597 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426598 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426599 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426600 GSE59086 25232098 GEO Tiling Array RNA
Experiment UCSC Genome Browser
Rv0981_B299 UCSC Browser Tracks
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.760000 1.34

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.986305
p-value INH: 0.988908
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 15.04 U
D3I 3 3 3.83 14.68 I
D3U 3 3 3.83 15.04 U
D5I 9 5 6.00 14.84 I
D5U 17 5 6.00 14.57 U
D7I 18 7 8.14 15.17 I
D7U 19 7 8.14 15.41 U
D14I 4 14 15.63 16.29 I
D14U 4 14 15.63 16.27 U
D17I 3 17 19.15 16.61 I
D17U 3 17 19.15 16.71 U
D21I 4 21 23.23 16.12 I
D21U 4 21 23.23 16.41 U
D24I 3 24 26.60 16.03 I
D24U 3 24 26.60 16.61 U
D28I 4 28 30.61 15.62 I
D28U 4 28 30.61 16.01 U