Rv0993 UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0993 galU UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) CDS 1109272 1110192 + 921 306 FALSE

Rv0993 (UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9)) is predicted to be co-regulated in modules bicluster_0033 with residual 0.56 and bicluster_0551 with residual 0.63.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0033 and 390.00 and 4,300.00 for bicluster_0551 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE UTP--GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE GALU [UDP-GLUCOSE PYROPHOSPHORYLASE] [UDPGP] [ALPHA-D-GLUCOSYL-1-PHOSPHATE URIDYLYLTRANSFERASE] [URIDINE DIPHOSPHOGLUCOSE PYROPHOSPHORYLASE] UTP--glucose-1-phosphate uridylyltransferase GalU
Operon # Operon
663
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Pentose and glucuronate interconversions

8
Total items in this category:  

KEGG

Galactose metabolism

14
Total items in this category:  

KEGG

Starch and sucrose metabolism

20
Total items in this category:  

KEGG

Amino sugar and nucleotide sugar metabolism

28
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608133 NP_215508.1 Run
GO:0003983

UTP:glucose-1-phosphate uridylyltransferase activity

UTP:glucose-1-phosphate uridylyltransferase activity

Details: 
Catalysis of the reaction: alpha-D-glucose 1-phosphate + UTP = diphosphate + UDP-D-glucose.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0003983

UTP:glucose-1-phosphate uridylyltransferase activity

UTP:glucose-1-phosphate uridylyltransferase activity

Details: 
Catalysis of the reaction: alpha-D-glucose 1-phosphate + UTP = diphosphate + UDP-D-glucose.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: