Rv0995 Ribosomal-protein-S5p-alanine acetyltransferase

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0995 rimJ Ribosomal-protein-S5p-alanine acetyltransferase CDS 1111612 1112223 + 612 203 FALSE

Rv0995 (Ribosomal-protein-S5p-alanine acetyltransferase) is predicted to be co-regulated in modules bicluster_0173 with residual 0.41 and bicluster_0443 with residual 0.45.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 11.00 and 12,000.00 for bicluster_0173 and 0.00 and 0.40 for bicluster_0443 respectively.

These modules are enriched for following go terms: cellular biosynthetic process, organic substance biosynthetic process, cytoplasm, RNA binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
POSSIBLE RIBOSOMAL-PROTEIN-ALANINE ACETYLTRANSFERASE RIMJ [ACETYLATING ENZYME FOR N-TERMINAL OF RIBOSOMAL PROTEIN S5] ribosomal-protein-alanine acetyltransferase
Operon # Operon
665
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608135 NP_215510.1 Run
GO:0008080

N-acetyltransferase activity

N-acetyltransferase activity

Details: 
Catalysis of the transfer of an acetyl group to a nitrogen atom on the acceptor molecule.
GO Category: 
molecular_function
3
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.010000 0.42

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: