Rv1007c Methionyl-tRNA synthetase (EC 6.1.1.10)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1007c metG Methionyl-tRNA synthetase (EC 6.1.1.10) CDS 1125444 1127003 - 1 560 519 FALSE

Rv1007c (Methionyl-tRNA synthetase (EC 6.1.1.10)) is predicted to be co-regulated in modules bicluster_0189 with residual 0.44 and bicluster_0190 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 13,000.00 and 13,000.00 for bicluster_0189 and 0.00 and 4.80 for bicluster_0190 respectively.

These modules are enriched for following go terms: glycerol metabolic process, alditol metabolic process, polyol metabolic process, alcohol metabolic process, organic hydroxy compound metabolic proce..., aspartic-type endopeptidase activity, aspartic-type peptidase activity, ATP-dependent DNA helicase activity, DNA-dependent ATPase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Methionyl-tRNA synthetase methionyl-tRNA synthetase
Operon # Operon
675
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Methionine--tRNA ligase Aminoacyl-tRNA biosynthesis, Selenoamino acid metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Selenocompound metabolism

40
Total items in this category:  

KEGG

Aminoacyl-tRNA biosynthesis

69
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608147 NP_215523.1 Run
GO:0004825

methionine-tRNA ligase activity

methionine-tRNA ligase activity

Details: 
Catalysis of the reaction: ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
GO Category: 
molecular_function
1
Total items in this category:  
GO:0004825

methionine-tRNA ligase activity

methionine-tRNA ligase activity

Details: 
Catalysis of the reaction: ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: