Rv1021 Nucleoside triphosphate pyrophosphohydrolase MazG (EC 3.6.1.8)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv1021 Nucleoside triphosphate pyrophosphohydrolase MazG (EC 3.6.1.8) CDS 1142671 1143648 + 978 325 FALSE

Rv1021 (Nucleoside triphosphate pyrophosphohydrolase MazG (EC 3.6.1.8)) is predicted to be co-regulated in modules bicluster_0055 with residual 0.58 and bicluster_0511 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.58 and 9.50 for bicluster_0055 and 670.00 and 6,900.00 for bicluster_0511 respectively.

These modules are enriched for following go terms: phosphopantetheine binding, modified amino acid binding, amide binding, amino acid binding.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:08
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18420 MT1049 2238
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein nucleoside triphosphate pyrophosphohydrolase
Operon # Operon
684 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

ATP diphosphatase Pyrimidine metabolism, Purine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Pyrimidine metabolism

41
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608161 NP_215537.1 Run
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.370000 1.06

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: