Rv1071c 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1071c echA9 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) CDS 1195055 1196092 - 1 038 345 FALSE

Rv1071c (3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4)) is predicted to be co-regulated in modules bicluster_0508 with residual 0.53 and bicluster_0569 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0508 and 500.00 and 460.00 for bicluster_0569 respectively.

These modules are enriched for following go terms: purine-nucleoside phosphorylase activity.

This gene is found to be essential for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:08
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18413 MT1101 2222
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0508
e.value: 
0.000000032
Motif Bicluster: 
e.value: 
0.0000025
Motif Bicluster: 
0.53
bicluster_0569
e.value: 
500
Motif Bicluster: 
e.value: 
460
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
POSSIBLE ENOYL-CoA HYDRATASE ECHA9 [ENOYL HYDRASE] [UNSATURATED ACYL-CoA HYDRATASE] [CROTONASE] enoyl-CoA hydratase
Operon # Operon
719 Rv1069c - Rv1070c - Rv1071c
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid metabolism

51
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Geraniol degradation

52
Total items in this category:  

KEGG

Lysine degradation

46
Total items in this category:  

KEGG

Benzoate degradation

48
Total items in this category:  

KEGG

Tryptophan metabolism

47
Total items in this category:  

KEGG

beta-Alanine metabolism

37
Total items in this category:  

KEGG

Aminobenzoate degradation

73
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608211 NP_215587.1 Run
GO:0003860

3-hydroxyisobutyryl-CoA hydrolase activity

3-hydroxyisobutyryl-CoA hydrolase activity

Details: 
Catalysis of the reaction: 3-hydroxy-2-methylpropanoyl-CoA + H2O = CoA + 3-hydroxy-2-methylpropanoate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
essential 0.000000 0.35

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.