Rv1085c COG1272: Predicted membrane protein hemolysin III homolog

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv1085c COG1272: Predicted membrane protein hemolysin III homolog CDS 1209756 1210484 - 729 242 FALSE

Rv1085c (COG1272: Predicted membrane protein hemolysin III homolog) is predicted to be co-regulated in modules bicluster_0403 with residual 0.53 and bicluster_0532 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0403 and 0.00 and 0.05 for bicluster_0532 respectively.

These modules are enriched for following go terms: cellular response to starvation, response to external stimulus, response to extracellular stimulus, response to nutrient levels, cellular response to extracellular stimu..., cellular response to nutrient levels, response to starvation, cellular response to external stimulus .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.22 -9 1210523 1210532 1210484
Last update: 10/16/2017 - 11:08
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18399 MT1117 2193
Product (LegacyBRC) Product (RefSeq)
UPF0073 membrane protein Rv1085c_MT1117 hemolysin-like protein
Operon # Operon
730
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608225 NP_215601.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.450000 0.45

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: