Rv1086 Undecaprenyl pyrophosphate synthetase (EC 2.5.1.31)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv1086 Undecaprenyl pyrophosphate synthetase (EC 2.5.1.31) CDS 1210595 1211383 + 789 262 FALSE

Rv1086 (Undecaprenyl pyrophosphate synthetase (EC 2.5.1.31)) is predicted to be co-regulated in modules bicluster_0158 with residual 0.52 and bicluster_0492 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 20,000.00 and 33,000.00 for bicluster_0158 and 4,900.00 and 7,700.00 for bicluster_0492 respectively.

These modules are enriched for following go terms: pyruvate dehydrogenase activity, pyruvate dehydrogenase (acetyl-transferr..., oxidoreductase activity, acting on the a... .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Short-chain Z-isoprenyl diphosphate synthetase short (C15) chain Z-isoprenyl diphosphate synthase (Z-FPP synthase) (Z-farnesyl diphosphate synthase) (Z-FPP synthetase) (Z-farnesyl diphosphate synthetase) (geranyltranstransferase) (farnesyl pyrophosphate synthetase)
Operon # Operon
731
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Di-trans,poly-cis-decaprenylcistransferase Terpenoid backbone biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Terpenoid backbone biosynthesis

27
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608226 NP_215602.1 Run
GO:0008834

di-trans,poly-cis-decaprenylcistransferase activity

di-trans,poly-cis-decaprenylcistransferase activity

Details: 
Catalysis of the reaction: di-trans-poly-cis-decaprenyl diphosphate + isopentenyl diphosphate = diphosphate + di-trans-poly-cis-undecaprenyl diphosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0000287

magnesium ion binding

magnesium ion binding

Details: 
Interacting selectively and non-covalently with magnesium (Mg) ions.
GO Category: 
molecular_function
52
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0016094

polyprenol biosynthetic process

polyprenol biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of polyprenols, prenols with more than 4 isoprenoid residues, which may be all-trans, or a mixture of cis and trans.
GO Category: 
biological_process
2
Total items in this category:  
GO:0030145

manganese ion binding

manganese ion binding

Details: 
Interacting selectively and non-covalently with manganese (Mn) ions.
GO Category: 
molecular_function
31
Total items in this category:  
GO:0033850

Z-farnesyl diphosphate synthase activity

Z-farnesyl diphosphate synthase activity

Details: 
Catalysis of the reaction: geranyl diphosphate + isopentenyl diphosphate = 2-cis,6-trans-farnesyl diphosphate + diphosphate.
GO Category: 
molecular_function
2
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.300000 11.45

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: