Rv1106c PROBABLE CHOLESTEROL DEHYDROGENASE

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv1106c PROBABLE CHOLESTEROL DEHYDROGENASE CDS 1232844 1233956 - 1 113 370 FALSE

Rv1106c (PROBABLE CHOLESTEROL DEHYDROGENASE) is predicted to be co-regulated in modules bicluster_0202 with residual 0.55 and bicluster_0245 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 150.00 for bicluster_0202 and 0.15 and 240.00 for bicluster_0245 respectively.

These modules are enriched for following go terms: oxidoreductase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-0.22 12 1233944 1233944 1233956
Last update: 10/16/2017 - 11:09
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-13636 MT1137 94
Product (LegacyBRC) Product (RefSeq)
PROBABLE CHOLESTEROL DEHYDROGENASE cholesterol dehydrogenase
Operon # Operon
748 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fructose and mannose metabolism

18
Total items in this category:  

KEGG

Bisphenol degradation

35
Total items in this category:  

KEGG

Linoleic acid metabolism

3
Total items in this category:  

KEGG

Chloroalkane and chloroalkene degradation

13
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608246 NP_215622.1 Run
GO:0003854

3-beta-hydroxy-delta5-steroid dehydrogenase activity

3-beta-hydroxy-delta5-steroid dehydrogenase activity

Details: 
Catalysis of the reaction: a 3-beta-hydroxy-delta(5)-steroid + NAD+ = a 3-oxo-delta(5)-steroid + NADH + H(+).
GO Category: 
molecular_function
1
Total items in this category:  
GO:0004769

steroid delta-isomerase activity

steroid delta-isomerase activity

Details: 
Catalysis of the reaction: a 3-oxo-delta(5)-steroid = a 3-oxo-delta(4)-steroid.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0070403

NAD+ binding

NAD+ binding

Details: 
Interacting selectively and non-covalently with the oxidized form, NAD, of nicotinamide adenine dinucleotide, a coenzyme involved in many redox and biosynthetic reactions.
GO Category: 
molecular_function
3
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.040000 0.53

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: