Rv1110 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1110 lytB 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2) CDS 1236185 1237192 + 1 008 335 FALSE

Rv1110 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2)) is predicted to be co-regulated in modules bicluster_0014 with residual 0.45 and bicluster_0029 with residual 0.45.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 950.00 and 4,900.00 for bicluster_0014 and 3.30 and 4.20 for bicluster_0029 respectively.

These modules are enriched for following go terms: nucleobase-containing compound catabolic..., aromatic compound catabolic process, macromolecule catabolic process, cellular nitrogen compound catabolic pro..., heterocycle catabolic process, organic cyclic compound catabolic proces..., deoxyribonuclease activity, exonuclease activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Rv1110 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Operon # Operon
749
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Terpenoid backbone biosynthesis

27
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116829 YP_177788.1 Run
GO:0051745

4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity

4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity

Details: 
Catalysis of the reaction: (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ = isopentenyl diphosphate + NAD(P)+ + H2O. Note that (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate is an alternative name for 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.
GO Category: 
molecular_function
2
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: