Rv1130 2-methylcitrate dehydratase (EC 4.2.1.79)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1130 prpD 2-methylcitrate dehydratase (EC 4.2.1.79) CDS 1254555 1256135 + 1 581 526 FALSE

Rv1130 (2-methylcitrate dehydratase (EC 4.2.1.79)) is predicted to be co-regulated in modules bicluster_0166 with residual 0.50 and bicluster_0191 with residual 0.46.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0166 and 0.27 and 2.30 for bicluster_0191 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.807 1254618 1254555
Last update: 10/16/2017 - 11:09
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14749 MT1162 1114
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
764 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

2-methylcitrate dehydratase Propanoate metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Propanoate metabolism

62
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608270 NP_215646.1 Run
GO:0047547

2-methylcitrate dehydratase activity

2-methylcitrate dehydratase activity

Details: 
Catalysis of the reaction: (2S,3S)-2-methylcitrate = cis-2-methylaconitate + H(2)O.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0001101

response to acid

response to acid

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an acid stimulus.
GO Category: 
biological_process
11
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0019629

propionate catabolic process, 2-methylcitrate cycle

propionate catabolic process, 2-methylcitrate cycle

Details: 
The chemical reactions and pathways resulting in the breakdown of propionate that occurs in the 2-methylcitrate cycle.
GO Category: 
biological_process
2
Total items in this category:  
GO:0019679

propionate metabolic process, methylcitrate cycle

propionate metabolic process, methylcitrate cycle

Details: 
The chemical reactions and pathways involving propionate that occur in the methylcitrate cycle.
GO Category: 
biological_process
2
Total items in this category:  
GO:0047547

2-methylcitrate dehydratase activity

2-methylcitrate dehydratase activity

Details: 
Catalysis of the reaction: (2S,3S)-2-methylcitrate = cis-2-methylaconitate + H(2)O.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.000000 0.01

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: