Rv1146 Transmembrane transport protein MmpL13b

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1146 mmpL13b Transmembrane transport protein MmpL13b CDS 1273355 1274767 + 1 413 470 FALSE

Rv1146 (Transmembrane transport protein MmpL13b) is predicted to be co-regulated in modules bicluster_0362 with residual 0.57 and bicluster_0442 with residual 0.47.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 32.00 and 150.00 for bicluster_0362 and 0.00 and 0.00 for bicluster_0442 respectively.

These modules are enriched for following go terms: nicotinamide nucleotide metabolic proces..., oxidoreduction coenzyme metabolic proces..., pyridine nucleotide metabolic process, glucose catabolic process, hexose catabolic process, monosaccharide catabolic process, pyridine-containing compound metabolic p..., organic substance catabolic process, catabolic process, oxidoreductase activity, acting on the C..., oxidoreductase activity, acting on CH-OH..., transferase activity, transferring aldeh....

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.269 1273265 1273355
Product (LegacyBRC) Product (RefSeq)
PROBABLE CONSERVED TRANSMEMBRANE TRANSPORT PROTEIN MMPL13B transmembrane transport protein MmpL13B
Operon # Operon
775 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608286 NP_215662.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.840000 1.57

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: