Rv1148c Partial REP13E12 repeat protein

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv1148c Partial REP13E12 repeat protein CDS 1276300 1277748 - 1 449 482 FALSE

Rv1148c (Partial REP13E12 repeat protein) is predicted to be co-regulated in modules bicluster_0452 with residual 0.53 and bicluster_0545 with residual 0.66.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0452 and 0.00 and 1.40 for bicluster_0545 respectively.

These modules are enriched for following go terms: aspartate-tRNA ligase activity monovalent inorganic cation transport, metal ion transport, cation-transporting ATPase activity, metal ion transmembrane transporter acti..., monovalent inorganic cation transmembran..., ATPase activity, coupled to transmembran....

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-0.016 84 1277664 1277664 1277748
Last update: 10/16/2017 - 11:09
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17810 MT1183 114
Product (LegacyBRC) Product (RefSeq)
Rv1148c
Operon # Operon
777
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608288 NP_215664.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.430000 1.29

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: