Rv1151c NAD-dependent protein deacetylase of SIR2 family

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv1151c NAD-dependent protein deacetylase of SIR2 family CDS 1278904 1279617 - 714 237 TRUE

Rv1151c (NAD-dependent protein deacetylase of SIR2 family) is predicted to be co-regulated in modules bicluster_0489 with residual 0.46 and bicluster_0537 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.10 and 6.50 for bicluster_0489 and 0.00 and 0.04 for bicluster_0537 respectively.

These modules are enriched for following go terms: response to stress, cellular response to stimulus, single-organism cellular process, nucleic acid metabolic process, four-way junction helicase activity, endodeoxyribonuclease activity, hydrolase activity, acting on ester bond....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
NAD-dependent deacetylase NAD-dependent deacetylase
Operon # Operon
779
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Amino sugar and nucleotide sugar metabolism

28
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608291 NP_215667.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1426617 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426618 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426619 GSE59086 25232098 GEO Tiling Array RNA
Experiment UCSC Genome Browser
Rv1151c_B434 UCSC Browser Tracks
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.290000 1.11

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.361302
p-value INH: 0.832328
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 5.47 U
D3I 3 3 3.83 4.32 I
D3U 3 3 3.83 3.22 U
D5I 9 5 6.00 I
D5U 17 5 6.00 U
D7I 18 7 8.14 3.87 I
D7U 19 7 8.14 4.44 U
D14I 4 14 15.63 I
D14U 4 14 15.63 3.40 U
D17I 3 17 19.15 2.30 I
D17U 3 17 19.15 U
D21I 4 21 23.23 2.16 I
D21U 4 21 23.23 U
D24I 3 24 26.60 I
D24U 3 24 26.60 U
D28I 4 28 30.61 I
D28U 4 28 30.61 1.73 U