Rv1152 Transcriptional regulator, GntR family

Product Feature Type Start End Strand Length AA Length is TF
Rv1152 Transcriptional regulator, GntR family CDS 1279655 1280020 + 366 121 TRUE

Rv1152 (Transcriptional regulator, GntR family) is predicted to be co-regulated in modules bicluster_0076 with residual 0.53 and bicluster_0537 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 3.40 for bicluster_0076 and 0.00 and 0.04 for bicluster_0537 respectively.

These modules are enriched for following go terms: response to stress, cellular response to stimulus, single-organism cellular process, nucleic acid metabolic process, four-way junction helicase activity, endodeoxyribonuclease activity, hydrolase activity, acting on ester bond....

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 11/20/2020 - 12:11
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14732 MT1186 410
Product (LegacyBRC) Product (RefSeq)
Operon # Operon
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Not assigned Not assigned
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15608292 NP_215668.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1426620 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426621 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426622 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426623 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426624 GSE59086 25232098 GEO Tiling Array RNA
Experiment UCSC Genome Browser
Rv1152_B182 UCSC Browser Tracks
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.200000 0.37

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.000168639
p-value INH: 0.883775
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 12.52 U
D3I 3 3 3.83 12.05 I
D3U 3 3 3.83 11.44 U
D5I 9 5 6.00 13.30 I
D5U 17 5 6.00 13.19 U
D7I 18 7 8.14 13.06 I
D7U 19 7 8.14 12.24 U
D14I 4 14 15.63 11.52 I
D14U 4 14 15.63 10.63 U
D17I 3 17 19.15 10.52 I
D17U 3 17 19.15 9.48 U
D21I 4 21 23.23 11.11 I
D21U 4 21 23.23 9.85 U
D24I 3 24 26.60 10.23 I
D24U 3 24 26.60 8.54 U
D28I 4 28 30.61 10.72 I
D28U 4 28 30.61 9.26 U