Rv1162 Respiratory nitrate reductase beta chain (EC 1.7.99.4)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1162 narH Respiratory nitrate reductase beta chain (EC 1.7.99.4) CDS 1291065 1292741 + 1 677 558 FALSE

Rv1162 (Respiratory nitrate reductase beta chain (EC 1.7.99.4)) is predicted to be co-regulated in modules bicluster_0154 with residual 0.56 and bicluster_0246 with residual 0.39.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 6.50 and 19,000.00 for bicluster_0154 and 890.00 and 3,200.00 for bicluster_0246 respectively.

These modules are enriched for following go terms: nitrate reductase complex, nitrate reductase activity, oxidoreductase activity, acting on other... macromolecular complex.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:09
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15109 MT1199 2463
Product (LegacyBRC) Product (RefSeq)
PROBABLE RESPIRATORY NITRATE REDUCTASE [BETA CHAIN] NARH respiratory nitrate reductase subunit beta NarH
Operon # Operon
788 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Nitrate reductase Nitrogen metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Nitrogen metabolism

24
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  

KEGG

Two-component system

53
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608302 NP_215678.1 Run
GO:0008940

nitrate reductase activity

nitrate reductase activity

Details: 
Catalysis of the reaction: nitrite + acceptor = nitrate + reduced acceptor.
GO Category: 
molecular_function
6
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.030000 1.14

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: