Rv1165 GTP-binding protein TypA/BipA

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1165 typA GTP-binding protein TypA/BipA CDS 1294168 1296054 + 1 887 628 FALSE

Rv1165 (GTP-binding protein TypA/BipA) is predicted to be co-regulated in modules bicluster_0154 with residual 0.56 and bicluster_0456 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 6.50 and 19,000.00 for bicluster_0154 and 190.00 and 9,400.00 for bicluster_0456 respectively.

These modules are enriched for following go terms: nitrate reductase complex, nitrate reductase activity, oxidoreductase activity, acting on other... .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:09
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14713 MT1202 108
Product (LegacyBRC) Product (RefSeq)
POSSIBLE GTP-BINDING TRANSLATION ELONGATION FACTOR TYPA [TYROSINE PHOSPHORYLATED PROTEIN A] [GTP-BINDING PROTEIN] GTP-binding translation elongation factor TypA
Operon # Operon
789 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608305 NP_215681.1 Run
GO:0005525

GTP binding

GTP binding

Details: 
Interacting selectively and non-covalently with GTP, guanosine triphosphate.
GO Category: 
molecular_function
18
Total items in this category:  
GO:0006412

translation

translation

Details: 
The cellular metabolic process in which a protein is formed, using the sequence of a mature mRNA molecule to specify the sequence of amino acids in a polypeptide chain. Translation is mediated by the ribosome, and begins with the formation of a ternary complex between aminoacylated initiator methionine tRNA, GTP, and initiation factor 2, which subsequently associates with the small subunit of the ribosome and an mRNA. Translation ends with the release of a polypeptide chain from the ribosome.
GO Category: 
biological_process
62
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.050000 0.75

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: