Rv1186c Regulator of polyketide synthase expression

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv1186c Regulator of polyketide synthase expression CDS 1327689 1329305 - 1 617 538 TRUE

Rv1186c (Regulator of polyketide synthase expression) is predicted to be co-regulated in modules bicluster_0017 with residual 0.60 and bicluster_0322 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.25 and 10.00 for bicluster_0017 and 0.00 and 350.00 for bicluster_0322 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 2 of 2
Gene Target Differential Expression Distance Expression pvalue Type
Regulator of polyketide synthase expression
Induced 37 3.72 2.58e-29 CDS
No 55 -0.62 0.324013 Primary.TSS
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Regulator of polyketide synthase expression
Induced 37 3.72 2.58e-29 CDS
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
807
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608326 NP_215702.1 Run
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1426631 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426632 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426633 GSE59086 25232098 GEO Tiling Array RNA
Experiment UCSC Genome Browser
Rv1186c_B611 UCSC Browser Tracks
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.910000 2.25

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.182549
p-value INH: 0.988908
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 14.07 U
D3I 3 3 3.83 14.08 I
D3U 3 3 3.83 13.83 U
D5I 9 5 6.00 14.78 I
D5U 17 5 6.00 14.68 U
D7I 18 7 8.14 13.84 I
D7U 19 7 8.14 13.88 U
D14I 4 14 15.63 10.95 I
D14U 4 14 15.63 12.31 U
D17I 3 17 19.15 8.28 I
D17U 3 17 19.15 10.91 U
D21I 4 21 23.23 8.39 I
D21U 4 21 23.23 11.41 U
D24I 3 24 26.60 5.42 I
D24U 3 24 26.60 10.36 U
D28I 4 28 30.61 5.78 I
D28U 4 28 30.61 11.02 U