Rv1187 Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1187 rocA Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12) CDS 1329390 1331021 + 1 632 543 FALSE

Rv1187 (Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)) is predicted to be co-regulated in modules bicluster_0322 with residual 0.56 and bicluster_0401 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 350.00 for bicluster_0322 and 100.00 and 180.00 for bicluster_0401 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:09
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14769 MT1224 84
Product (LegacyBRC) Product (RefSeq)
PROBABLE PYRROLINE-5-CARBOXYLATE DEHYDROGENASE ROCA pyrroline-5-carboxylate dehydrogenase ROCA
Operon # Operon
808 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Alanine, aspartate and glutamate metabolism

26
Total items in this category:  

KEGG

Arginine and proline metabolism

38
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608327 NP_215703.1 Run
GO:0003842

1-pyrroline-5-carboxylate dehydrogenase activity

1-pyrroline-5-carboxylate dehydrogenase activity

Details: 
Catalysis of the reaction: 1-pyrroline-5-carboxylate + NAD+ + H2O = L-glutamate + NADH + H(+).
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.860000 1.50

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: