Rv1213 Glucose-1-phosphate adenylyltransferase (EC 2.7.7.27)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1213 glgC Glucose-1-phosphate adenylyltransferase (EC 2.7.7.27) CDS 1355836 1357050 + 1 215 404 FALSE

Rv1213 (Glucose-1-phosphate adenylyltransferase (EC 2.7.7.27)) is predicted to be co-regulated in modules bicluster_0221 with residual 0.50 and bicluster_0549 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 15.00 and 780.00 for bicluster_0221 and 0.28 and 340.00 for bicluster_0549 respectively.

These modules are enriched for following go terms: monocarboxylic acid binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Glucose-1-phosphate adenylyltransferase glucose-1-phosphate adenylyltransferase
Operon # Operon
827
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Starch and sucrose metabolism

20
Total items in this category:  

KEGG

Amino sugar and nucleotide sugar metabolism

28
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608353 NP_215729.1 Run
GO:0008878

glucose-1-phosphate adenylyltransferase activity

glucose-1-phosphate adenylyltransferase activity

Details: 
Catalysis of the reaction: alpha-D-glucose 1-phosphate + ATP = ADP-glucose + diphosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0009250

glucan biosynthetic process

glucan biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of glucans, polysaccharides consisting only of glucose residues.
GO Category: 
biological_process
3
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.470000 1.50

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: