Rv1266c Serine/threonine protein kinase (EC 2.7.11.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1266c pknH Serine/threonine protein kinase (EC 2.7.11.1) CDS 1413960 1415840 - 1 881 626 FALSE

Rv1266c (Serine/threonine protein kinase (EC 2.7.11.1)) is predicted to be co-regulated in modules bicluster_0122 with residual 0.57 and bicluster_0535 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1.10 and 6.50 for bicluster_0122 and 11.00 and 3,000.00 for bicluster_0535 respectively.

These modules are enriched for following go terms: cellular protein modification process, protein modification process, phosphorylation, protein kinase activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:16
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18683 MT1304 2834
Product (LegacyBRC) Product (RefSeq)
Probable serine_threonine-protein kinase pknH transmembrane serine/threonine-protein kinase H
Operon # Operon
861
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608406 NP_215782.1 Run
GO:0004672

protein kinase activity

protein kinase activity

Details: 
Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
GO Category: 
molecular_function
18
Total items in this category:  
GO:0004674

protein serine/threonine kinase activity

protein serine/threonine kinase activity

Details: 
Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
GO Category: 
molecular_function
10
Total items in this category:  
GO:0005887

integral to plasma membrane

integral to plasma membrane

Details: 
Penetrating at least one phospholipid bilayer of a plasma membrane. May also refer to the state of being buried in the bilayer with no exposure outside the bilayer.
GO Category: 
cellular_component
101
Total items in this category:  
GO:0006950

response to stress

response to stress

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a disturbance in organismal or cellular homeostasis, usually, but not necessarily, exogenous (e.g. temperature, humidity, ionizing radiation).
GO Category: 
biological_process
9
Total items in this category:  
GO:0043085

positive regulation of catalytic activity

positive regulation of catalytic activity

Details: 
Any process that activates or increases the activity of an enzyme.
GO Category: 
biological_process
7
Total items in this category:  
GO:0043086

negative regulation of catalytic activity

negative regulation of catalytic activity

Details: 
Any process that stops or reduces the activity of an enzyme.
GO Category: 
biological_process
7
Total items in this category:  
GO:0043388

positive regulation of DNA binding

positive regulation of DNA binding

Details: 
Any process that increases the frequency, rate or extent of DNA binding. DNA binding is any process in which a gene product interacts selectively with DNA (deoxyribonucleic acid).
GO Category: 
biological_process
3
Total items in this category:  
GO:0045926

negative regulation of growth

negative regulation of growth

Details: 
Any process that stops, prevents or reduces the rate or extent of growth, the increase in size or mass of all or part of an organism.
GO Category: 
biological_process
36
Total items in this category:  
GO:0045893

positive regulation of transcription, DNA-dependent

positive regulation of transcription, DNA-dependent

Details: 
Any process that activates or increases the frequency, rate or extent of cellular DNA-dependent transcription.
GO Category: 
biological_process
14
Total items in this category:  
GO:0046777

protein autophosphorylation

protein autophosphorylation

Details: 
The phosphorylation by a protein of one or more of its own amino acid residues, or residues on an identical protein.
GO Category: 
biological_process
21
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.070000 1.20

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: