Rv1267c Transcriptional regulatory protein EmbR

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1267c embR Transcriptional regulatory protein EmbR CDS 1416181 1417347 - 1 167 388 FALSE

Rv1267c (Transcriptional regulatory protein EmbR) is predicted to be co-regulated in modules bicluster_0044 with residual 0.54 and bicluster_0319 with residual 0.47.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2.50 and 3.40 for bicluster_0044 and 0.16 and 8.30 for bicluster_0319 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 11/20/2020 - 12:11
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-13637 MT1305 66
Product (LegacyBRC) Product (RefSeq)
Probable regulatory protein embR transcriptional regulatory protein EMBR
Operon # Operon
862
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608407 NP_215783.1 Run
GO:0003677

DNA binding

DNA binding

Details: 
Any molecular function by which a gene product interacts selectively with DNA (deoxyribonucleic acid).
GO Category: 
molecular_function
37
Total items in this category:  
GO:0003924

GTPase activity

GTPase activity

Details: 
Catalysis of the reaction: GTP + H2O = GDP + phosphate.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0016887

ATPase activity

ATPase activity

Details: 
Catalysis of the reaction: ATP + H2O = ADP + phosphate + 2 H+. May or may not be coupled to another reaction.
GO Category: 
molecular_function
25
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.120000 0.98

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.510348
p-value INH: 0.959103
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 13.39 U
D3I 3 3 3.83 12.85 I
D3U 3 3 3.83 12.87 U
D5I 9 5 6.00 12.97 I
D5U 17 5 6.00 12.79 U
D7I 18 7 8.14 13.27 I
D7U 19 7 8.14 13.70 U
D14I 4 14 15.63 14.68 I
D14U 4 14 15.63 14.50 U
D17I 3 17 19.15 14.81 I
D17U 3 17 19.15 14.87 U
D21I 4 21 23.23 14.02 I
D21U 4 21 23.23 14.45 U
D24I 3 24 26.60 13.83 I
D24U 3 24 26.60 14.73 U
D28I 4 28 30.61 13.17 I
D28U 4 28 30.61 14.01 U