Rv1286 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1286 cysN Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) CDS 1438907 1440751 + 1 845 614 FALSE

Rv1286 (Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25)) is predicted to be co-regulated in modules bicluster_0023 with residual 0.49 and bicluster_0546 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 200.00 and 300.00 for bicluster_0023 and 0.00 and 0.21 for bicluster_0546 respectively.

These modules are enriched for following go terms: adenylyltransferase activity, transferase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Bifunctional enzyme cysN_cysC bifunctional sulfate adenylyltransferase subunit 1/adenylylsulfate kinase protein
Operon # Operon
873 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Selenocompound metabolism

40
Total items in this category:  

KEGG

Sulfur metabolism

12
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608426 NP_215802.1 Run
GO:0004020

adenylylsulfate kinase activity

adenylylsulfate kinase activity

Details: 
Catalysis of the reaction: ATP + adenylylsulfate = ADP + 3'-phosphoadenosine 5'-phosphosulfate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0009336

sulfate adenylyltransferase complex (ATP)

sulfate adenylyltransferase complex (ATP)

Details: 
An enzyme complex that catalyzes the formation adenylylsulfate from sulfate and ATP.
GO Category: 
cellular_component
2
Total items in this category:  
GO:0010134

sulfate assimilation via adenylyl sulfate reduction

sulfate assimilation via adenylyl sulfate reduction

Details: 
The pathway by which inorganic sulfate is activated, reduced and incorporated into sulfated compounds, where the activated sulfate, adenylyl-sulfate, is reduced to sulfite by the activity of adenylyl-sulfate reductase.
GO Category: 
biological_process
2
Total items in this category:  
GO:0010438

cellular response to sulfur starvation

cellular response to sulfur starvation

Details: 
Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of sulfur.
GO Category: 
biological_process
2
Total items in this category:  
GO:0034599

cellular response to oxidative stress

cellular response to oxidative stress

Details: 
Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of oxidative stress, a state often resulting from exposure to high levels of reactive oxygen species, e.g. superoxide anions, hydrogen peroxide (H2O2), and hydroxyl radicals.
GO Category: 
biological_process
4
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.500000 0.03

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: