Rv1302 Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1302 rfe Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-) CDS 1458295 1459509 + 1 215 404 FALSE

Rv1302 (Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-)) is predicted to be co-regulated in modules bicluster_0317 with residual 0.52 and bicluster_0506 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 12,000.00 and 12,000.00 for bicluster_0317 and 0.02 and 2.50 for bicluster_0506 respectively.

These modules are enriched for following go terms: flavin adenine dinucleotide binding .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:16
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17936 MT1341 533
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 6 of 6
ChipSeq TF Differential Expression Distance Expression pvalue Type
DNA-binding response regulator
Induced -2 1.51 1.47e-25 CDS
transcriptional regulator, ArsR family
Induced -42 0.65 0.0000000209 Internal.TSS
DNA-binding response regulator
Induced -20 1.51 1.47e-25 Internal.TSS
\Transcriptional regulator, XRE family\\\""
No -40 0.36 0.0536318 CDS
transcriptional regulator, ArsR family
Induced -24 0.65 0.0000000209 CDS
\Transcriptional regulator, XRE family\\\""
No -58 0.36 0.0536318 Internal.TSS
Motif 1 Motif 2 Residual
bicluster_0317
e.value: 
12000
Motif Bicluster: 
e.value: 
12000
Motif Bicluster: 
0.52
bicluster_0506
e.value: 
0.018
Motif Bicluster: 
e.value: 
2.5
Motif Bicluster: 
0.55
Product (LegacyBRC) Product (RefSeq)
Putative undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase undecapaprenyl-phosphate alpha-N-acetylglucosaminyltransferase rfe (UDP-GlcNAc transferase)
Operon # Operon
885
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608442 NP_215818.1 Run
GO:0003824

catalytic activity

catalytic activity

Details: 
Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO Category: 
molecular_function
12
Total items in this category:  
GO:0006629

lipid metabolic process

lipid metabolic process

Details: 
The chemical reactions and pathways involving lipids, compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent. Includes fatty acids; neutral fats, other fatty-acid esters, and soaps; long-chain (fatty) alcohols and waxes; sphingoids and other long-chain bases; glycolipids, phospholipids and sphingolipids; and carotenes, polyprenols, sterols, terpenes and other isoprenoids.
GO Category: 
biological_process
3
Total items in this category:  
GO:0016020

membrane

membrane

Details: 
Double layer of lipid molecules that encloses all cells, and, in eukaryotes, many organelles; may be a single or double lipid bilayer; also includes associated proteins.
GO Category: 
cellular_component
33
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: