Rv1314c Cob(I)alamin adenosyltransferase PduO (EC 2.5.1.17)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv1314c Cob(I)alamin adenosyltransferase PduO (EC 2.5.1.17) CDS 1469671 1470252 - 582 193 FALSE

Rv1314c (Cob(I)alamin adenosyltransferase PduO (EC 2.5.1.17)) is predicted to be co-regulated in modules bicluster_0023 with residual 0.49 and bicluster_0077 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 200.00 and 300.00 for bicluster_0023 and 37.00 and 12,000.00 for bicluster_0077 respectively.

These modules are enriched for following go terms: adenylyltransferase activity, transferase activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:16
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18501 MT1354 2409
Product (LegacyBRC) Product (RefSeq)
Cob[I]yrinic acid ac-diamide adenosyltransferase
Operon # Operon
890
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608454 NP_215830.1 Run
GO:0008817

cob(I)yrinic acid a,c-diamide adenosyltransferase activity

cob(I)yrinic acid a,c-diamide adenosyltransferase activity

Details: 
Catalysis of the reaction: ATP + cob(I)alamin + H2O = phosphate + diphosphate + adenosylcobalamin.
GO Category: 
molecular_function
2
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.460000 1.40

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: