Rv1318c Adenylate cyclase (EC 4.6.1.1)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv1318c Adenylate cyclase (EC 4.6.1.1) CDS 1479199 1480824 - 1 626 541 FALSE

Rv1318c (Adenylate cyclase (EC 4.6.1.1)) is predicted to be co-regulated in modules bicluster_0069 with residual 0.54 and bicluster_0261 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0069 and 0.03 and 260.00 for bicluster_0261 respectively.

These modules are enriched for following go terms: S-methyltransferase activity, signal transducer activity, molecular transducer activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:16
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15671 MT1359 1491
Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv1318c_MT1359 adenylate cyclase
Operon # Operon
893
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Adenylate cyclase Purine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608458 NP_215834.1 Run
GO:0004016

adenylate cyclase activity

adenylate cyclase activity

Details: 
Catalysis of the reaction: ATP = 3',5'-cyclic AMP + diphosphate.
GO Category: 
molecular_function
11
Total items in this category:  
GO:0004016

adenylate cyclase activity

adenylate cyclase activity

Details: 
Catalysis of the reaction: ATP = 3',5'-cyclic AMP + diphosphate.
GO Category: 
molecular_function
11
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.290000 1.40

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: