Rv1320c Adenylate cyclase (EC 4.6.1.1)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv1320c Adenylate cyclase (EC 4.6.1.1) CDS 1482514 1484217 - 1 704 567 FALSE

Rv1320c (Adenylate cyclase (EC 4.6.1.1)) is predicted to be co-regulated in modules bicluster_0261 with residual 0.52 and bicluster_0443 with residual 0.45.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.03 and 260.00 for bicluster_0261 and 0.00 and 0.40 for bicluster_0443 respectively.

These modules are enriched for following go terms: S-methyltransferase activity, signal transducer activity, molecular transducer activity cellular biosynthetic process, organic substance biosynthetic process, cytoplasm, RNA binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv1320c_MT1362 adenylate cyclase
Operon # Operon
894 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Adenylate cyclase Purine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608460 NP_215836.1 Run
GO:0004016

adenylate cyclase activity

adenylate cyclase activity

Details: 
Catalysis of the reaction: ATP = 3',5'-cyclic AMP + diphosphate.
GO Category: 
molecular_function
11
Total items in this category:  
GO:0004016

adenylate cyclase activity

adenylate cyclase activity

Details: 
Catalysis of the reaction: ATP = 3',5'-cyclic AMP + diphosphate.
GO Category: 
molecular_function
11
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.590000 1.51

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: