Rv1326c 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1326c glgB 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18) CDS 1490117 1492312 - 2 196 731 FALSE

Rv1326c (1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)) is predicted to be co-regulated in modules bicluster_0473 with residual 0.49 and bicluster_0508 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0473 and 0.00 and 0.00 for bicluster_0508 respectively.

These modules are enriched for following go terms: protein binding, carboxypeptidase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
14-alpha-glucan-branching enzyme glycogen branching enzyme
Operon # Operon
900 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

1,4-alpha-glucan branching enzyme Starch and sucrose metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Starch and sucrose metabolism

20
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608466 NP_215842.1 Run
GO:0003844

1,4-alpha-glucan branching enzyme activity

1,4-alpha-glucan branching enzyme activity

Details: 
Catalysis of the transfer of a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxyl group in a similar glucan chain.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0003844

1,4-alpha-glucan branching enzyme activity

1,4-alpha-glucan branching enzyme activity

Details: 
Catalysis of the transfer of a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxyl group in a similar glucan chain.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0005978

glycogen biosynthetic process

glycogen biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of glycogen, a polydisperse, highly branched glucan composed of chains of D-glucose residues.
GO Category: 
biological_process
3
Total items in this category:  
GO:0009250

glucan biosynthetic process

glucan biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of glucans, polysaccharides consisting only of glucose residues.
GO Category: 
biological_process
3
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: