Rv1328 Glycogen phosphorylase (EC

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1328 glgP Glycogen phosphorylase (EC CDS 1494564 1497155 + 2 592 863 FALSE

Rv1328 (Glycogen phosphorylase (EC is predicted to be co-regulated in modules bicluster_0473 with residual 0.49 and bicluster_0508 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0473 and 0.00 and 0.00 for bicluster_0508 respectively.

These modules are enriched for following go terms: protein binding, carboxypeptidase activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:16
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15711 MT1370 1581
Product (LegacyBRC) Product (RefSeq)
Glycogen phosphorylase glycogen phosphorylase GlgP
Operon # Operon
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Phosphorylase Starch and sucrose metabolism
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


Starch and sucrose metabolism

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BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15608468 NP_215844.1 Run

phosphorylase activity

phosphorylase activity

Catalysis of the reaction: 1,4-alpha-D-glucosyl(n) + phosphate = 1,4-alpha-D-glucosyl(n-1) + alpha-D-glucose 1-phosphate. The name should be qualified in each instance by adding the name of the natural substrate, e.g. maltodextrin phosphorylase, starch phosphorylase, glycogen phosphorylase.
GO Category: 
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The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
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plasma membrane

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
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No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.640000 1.32

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: