Rv1330c Nicotinate phosphoribosyltransferase (EC 2.4.2.11)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1330c pncB1 Nicotinate phosphoribosyltransferase (EC 2.4.2.11) CDS 1499213 1500559 - 1 347 448 FALSE

Rv1330c (Nicotinate phosphoribosyltransferase (EC 2.4.2.11)) is predicted to be co-regulated in modules bicluster_0136 with residual 0.42 and bicluster_0482 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0136 and 0.02 and 0.93 for bicluster_0482 respectively.

These modules are enriched for following go terms: cellular macromolecule biosynthetic proc..., macromolecule biosynthetic process, macromolecule metabolic process, cytoplasm.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.448 1500637 1500559
Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv1330c_MT1372 nicotinate phosphoribosyltransferase
Operon # Operon
902 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Nicotinate phosphoribosyltransferase Nicotinate and nicotinamide metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Nicotinate and nicotinamide metabolism

16
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116850 NP_215846.2 Run
GO:0004516

nicotinate phosphoribosyltransferase activity

nicotinate phosphoribosyltransferase activity

Details: 
Catalysis of the reaction: diphosphate + nicotinate D-ribonucleotide = 5-phospho-alpha-D-ribose 1-diphosphate + H(+) + nicotinate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0004516

nicotinate phosphoribosyltransferase activity

nicotinate phosphoribosyltransferase activity

Details: 
Catalysis of the reaction: diphosphate + nicotinate D-ribonucleotide = 5-phospho-alpha-D-ribose 1-diphosphate + H(+) + nicotinate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0009435

NAD biosynthetic process

NAD biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of nicotinamide adenine dinucleotide, a coenzyme present in most living cells and derived from the B vitamin nicotinic acid; biosynthesis may be of either the oxidized form, NAD, or the reduced form, NADH.
GO Category: 
biological_process
4
Total items in this category:  
GO:0034355

NAD salvage

NAD salvage

Details: 
Any process that generates nicotinamide adenine dinucleotide (NAD) from derivatives of it, without de novo synthesis; salvage is usually from the degradation products nicotinic acid (Na) and nicotinamide (Nam).
GO Category: 
biological_process
2
Total items in this category:  
GO:0047280

nicotinamide phosphoribosyltransferase activity

nicotinamide phosphoribosyltransferase activity

Details: 
Catalysis of the reaction: diphosphate + nicotinamide mononucleotide = 5-phospho-alpha-D-ribose 1-diphosphate + H(+) + nicotinamide.
GO Category: 
molecular_function
2
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.100000 0.95

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: