Rv1344 Acyl carrier protein MbtL

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1344 mbtL Acyl carrier protein MbtL CDS 1508968 1509288 + 321 106 FALSE

Rv1344 (Acyl carrier protein MbtL) is predicted to be co-regulated in modules bicluster_0446 with residual 0.47 and bicluster_0502 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,700.00 and 5,500.00 for bicluster_0446 and 0.00 and 1.20 for bicluster_0502 respectively.

These modules are enriched for following go terms: .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR Re-Annotated Start Tuberculist Annotated Start
-0.698 108 1509076 -42 1509034 1508968
Last update: 10/16/2017 - 11:16
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14792 MT1385 1680
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator kstR (Rv3574), TetR family
Induced -141 0.66 0.0111534 CDS
Motif 1 Motif 2 Residual
bicluster_0446
e.value: 
1700
Motif Bicluster: 
e.value: 
5500
Motif Bicluster: 
0.47
bicluster_0502
e.value: 
2e-16
Motif Bicluster: 
e.value: 
1.2
Motif Bicluster: 
0.52
Product (LegacyBRC) Product (RefSeq)
Acyl carrier protein mbtL acyl carrier protein
Operon # Operon
905 Rv1344 - Rv1345 - Rv1346
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608484 NP_215860.1 Run
GO:0000036

ACP phosphopantetheine attachment site binding involved in fatty acid biosynthetic process

ACP phosphopantetheine attachment site binding involved in fatty acid biosynthetic process

Details: 
Interacting selectively with the attachment site of the phosphopantetheine prosthetic group of an acyl carrier protein (ACP) as part of the process of fatty acid biosynthesis.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.620000 1.28

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.